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DIN EN ISO 15216-1:2021-10

DIN EN ISO 15216-1:2021-10

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017 + Amd 1:2021); German version EN ISO 15216-1:2017 + A1:2021

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche des virus de l'hépatite A et norovirus par la technique RT-PCR en temps réel - Partie 1: Méthode de quantification (ISO 15216-1:2017 + Amd 1:2021); Version allemande EN ISO 15216-1:2017 + A1:2021

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zur Bestimmung von Hepatitis-A-Virus und Norovirus mittels Real-time-RT-PCR - Teil 1: Verfahren zur Quantifizierung (ISO 15216-1:2017 + Amd 1:2021); Deutsche Fassung EN ISO 15216-1:2017 + A1:2021

Date:
2021-10 /Active
Idiomas Disponibles:
Inglés, Alemán
ICS:

07.100.30 - Food microbiology

International equivalences:

EN ISO 15216-1 (2017-03)

EN ISO 15216-1/A1 (2021-04)

ISO 15216-1 (2017-03)

ISO 15216-1 AMD 1 (2021-03)

BVL L 00.00-147/1 (2022-04)

Relación con otras normas DIN:

Reemplaza a: DIN EN ISO 15216-1 (2017-07)

Reemplaza a: DIN EN ISO 15216-1/A1 (2020-09)

Summary:
This document specifies a method for quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.
Keywords:
Animal feed, Bacterial viruses, Chemical reactions, Contamination, Definitions, Food inspection, Food products, Food testing, Foods, Foodstuff, Hepatitis, Infections, Investigations, Laboratory testing, Laboratory tests, Methods of analysis, Microbial foods, Microbiological analysis, Microbiology, Microorganisms, Noroviruses, PCR, Polymerase, Polymerase chain reaction, Quantitative analysis, Real-time, Sampling methods, Testing, Verification, Viruses
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