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DIN EN ISO 15216-2:2019-12

DIN EN ISO 15216-2:2019-12

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 2: Method for detection (ISO 15216-2:2019); German version EN ISO 15216-2:2019

Microbiologie dans la chaine alimentaire - Méthode horizontale pour la recherche des virus de l'hépatite A et norovirus par la technique RT-PCR en temps réel - Partie 2: Méthode de détection (ISO 15216-2:2019); Version allemande EN ISO 15216-2:2019

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zur Bestimmung von Hepatitis-A-Virus und Norovirus in Lebensmitteln mittels Real-time-RT-PCR - Teil 2: Nachweisverfahren (ISO 15216-2:2019); Deutsche Fassung EN ISO 15216-2:2019

Date:
2019-12 /Active
Idiomas Disponibles:
Inglés, Alemán
ICS:

07.100.30 - Food microbiology

International equivalences:

EN ISO 15216-2 (2019-09)

ISO 15216-2 (2019-07)

BVL L 12.03-1 (2021-07)

Summary:
This document specifies a method for detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
Keywords:
Animal feed, Bacterial viruses, Contamination, Definitions, Food inspection, Food products, Food testing, Hepatitis, Investigations, Laboratory testing, Laboratory tests, Microbial foods, Microbiological analysis, Microorganisms, Noroviruses, PCR, Polymerase, Polymerase chain reaction, Qualitative analysis, Real-time, Surfaces, Verification, Viruses
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